LOCUS pGS201 6062 bp DNA circular 13-APR-2007 DEFINITION attR1-attR2. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT VNTDATE|443532415| COMMENT VNTDBDATE|443532415| COMMENT LSOWNER| COMMENT VNTNAME|pGS201| COMMENT VNTAUTHORNAME|Scott Nolan| COMMENT VNTAUTHOREML|snolan6@jhmi.edu| COMMENT VNTAUTHORAD1|1550 Orleans St. (CRB2) Rm 176| FEATURES Location/Qualifiers misc_recomb complement(5935..6059) /vntifkey="86" /label=attR1 CDS complement(5167..5826) /vntifkey="4" /label=Cm(R) CDS complement(4520..4825) /vntifkey="4" /label=ccdB misc_recomb 4355..4479 /vntifkey="86" /label=attR2 misc_recomb 527..569 /vntifkey="86" /label=attP CDS 854..1852 /vntifkey="4" /label=int /note="Mycobacteriophage L5 integrase" rep_origin complement(3638..4252) /vntifkey="33" /label=oriE /note="E. coli origin of replication" CDS 2357..3172 /vntifkey="4" /label=Km(R) /note="Kanamycin resistance" BASE COUNT 1475 a 1574 c 1603 g 1410 t ORIGIN 1 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag 61 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt 121 gtaaaacgac ggccagtgaa ttcgagctcg gtacccgggg atcctctaga gtcgaccacc 181 aagggcacca tctctgcttg ggccaccccg ttggccgcag ccagctcgct gagagccgtg 241 aacgacaggg cgaacgccag cccgccgacg gcgagggttc cgaccgctgc aactcccggt 301 gcaaccttgt cccggtctat tctcttcact gcaccagctc caatctggtg tgaatgcccc 361 tcgtctgttc gcgcaggcgg ggggctctat tcgtttgtca gcatcgaaag tagccagatc 421 agggatgcgt tgcaaccgcg tatgcccagg tcagaagagt cgcacaagag ttgcagaccc 481 ctggaaagaa aaatggccag agggcgaaaa caccctctga ccagcggagc gggcgacggg 541 aatcgaaccc gcgtagctag tttggaagaa tgggtgtctg ccgaccacat atgggccggt 601 caagataggt ttttaccccc tctcggctgc atcctctaag tggaaagaaa ttgcaggtcg 661 tagaagcgcg ttgaagcctg agagttgcac aggagttgca acccggtagc cttgttcacg 721 acgagaggag acctagttgg cacgtcgcgg atggggatcg ctgaagactc agcgcagcgg 781 gaggatccaa gcctcatacg tcaacccgca ggacggtgtg aggtactacg cgctgcagac 841 ctacgacaac aagatggacg ccgaagcctg gctcgcgggc gagaagcggc tcatcgagat 901 ggagacctgg acccctccac aggaccgggc gaagaaggca gccgccagcg ccatcacgct 961 ggaggagtac acccggaagt ggctcgtgga gcgcgacctc gcagacggca ccagggatct 1021 gtacagcggg cacgcggagc gccgcatcta cccggtgcta ggtgaagtgg cggtcacaga 1081 gatgacgcca gctctggtgc gtgcgtggtg ggccgggatg ggtaggaagc acccgactgc 1141 ccgccggcat gcctacaacg tcctccgggc ggtgatgaac acagcggtcg aggacaagct 1201 gatcgcagag aacccgtgcc ggatcgagca gaaggcagcc gatgagcgcg acgtagaggc 1261 gctgacgcct gaggagctgg acatcgtcgc cgctgagatc ttcgagcact accggatcgc 1321 ggcatacatc ctggcgtgga cgagcctccg gttcggagag ctgatcgagc ttcgccgcaa 1381 ggacatcgtg gacgacggca tgacgatgaa gctccgggtg cgccgtggcg cttcccgcgt 1441 ggggaacaag atcgtcgttg gcaacgccaa gaccgtccgg tcgaagcgtc ctgtgacggt 1501 tccgcctcac gtcgcggaga tgatccgagc gcacatgaag gaccgtacga agatgaacaa 1561 gggccccgag gcattcctgg tgaccacgac gcagggcaac cggctgtcga agtccgcgtt 1621 caccaagtcg ctgaagcgtg gctacgccaa gatcggtcgg ccggaactcc gcatccacga 1681 cctccgcgct gtcggcgcta cgttcgccgc tcaggcaggt gcgacgacca aggagctgat 1741 ggcccgtctc ggtcacacga ctcctaggat ggcgatgaag taccagatgg cgtctgaggc 1801 ccgcgacgag gctatcgctg aggcgatgtc caagctggcc aagacctcct gaaacgcaaa 1861 aagcccccct cccaaggaca ctgagtccta aagagggggg tttcttgtca gtacgcgaag 1921 aaccacgcct ggccgcgagc gccagcaccg ccgctctgtg cggagacctg ggcaccagcc 1981 ccgccgccgc caggagcatt gccgttcccg ccagctgagt tctgttgtgc gccgcctatg 2041 tagagctggt cgttgtaggt ccgatctcca ggcgactttc cggcgacgct gaggatgtcg 2101 atcacagagc ctccgggacc gccggttgcg gtcaaacctg accatccgac agcggacgcc 2161 gtggtgtttc ctccagggcc tccggccttg cctgagaata cagagccagc tcccgctgcg 2221 cctccagctc cgacgagccc ggtgatcgtc ttggtcgacc tgcaggcatg caagctttta 2281 cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag 2341 taatacaagg ggtgttatga gccatattca acgggaaacg tcttgctcga ggccgcgatt 2401 aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca 2461 atcaggtgcg acaatctatc gattgtatgg gaagcccgat gcgccagagt tgtttctgaa 2521 acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct 2581 gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg 2641 gttactcacc actgcgatcc ccgggaaaac agcattccag gtattagaag aatatcctga 2701 ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc 2761 tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg 2821 aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt 2881 tgaacaagtc tggaaagaaa tgcataaact tttgccattc tcaccggatt cagtcgtcac 2941 tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat 3001 tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg 3061 cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa 3121 tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt 3181 ggttaattgg ttgtaacact ggcagagcat tacgctgact tgacgggacg gcaagcttgg 3241 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca 3301 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca 3361 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc 3421 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt 3481 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 3541 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag 3601 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata 3661 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 3721 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 3781 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 3841 tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 3901 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 3961 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 4021 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 4081 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 4141 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 4201 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt 4261 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat 4321 tatcaaaaag gatcttcacc tagatccttt tatcaccact ttgtacaaga aagctgaacg 4381 agaaacgtaa aatgatataa atatcaatat attaaattag attttgcata aaaaacagac 4441 tacataatgc tgtaaaacac aacatatcca gtcactatgg tcgacctgca gactggctgt 4501 gtataaggga gcctgacatt tatattcccc agaacatcag gttaatggcg tttttgatgt 4561 cattttcgcg gtggctgaga tcagccactt cttccccgat aacggagacc ggcacactgg 4621 ccatatcggt ggtcatcatg cgccagcttt catccccgat atgcaccacc gggtaaagtt 4681 cacgggagac tttatctgac agcagacgtg cactggccag ggggatcacc atccgtcgcc 4741 cgggcgtgtc aataatatca ctctgtacat ccacaaacag acgataacgg ctctctcttt 4801 tataggtgta aaccttaaac tgcatttcac cagcccctgt tctcgtcagc aaaagagccg 4861 ttcatttcaa taaaccgggc gacctcagcc atcccttcct gattttccgc tttccagcgt 4921 tcggcacgca gacgacgggc ttcattctgc atggttgtgc ttaccagacc ggagatattg 4981 acatcatata tgccttgagc aactgatagc tgtcgctgtc aactgtcact gtaatacgct 5041 gcttcatagc atacctcttt ttgacatact tcgggtatac atatcagtat atattcttat 5101 accgcaaaaa tcagcgcgca aatacgcata ctgttatctg gcttttagta agccggatcc 5161 acgcgtttac gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct 5221 gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac 5281 cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat 5341 attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg agacgaaaaa 5401 catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc 5461 ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga 5521 aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac 5581 cagctcaccg tctttcattg ccatacggaa ttccggatga gcattcatca ggcgggcaag 5641 aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc 5701 cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc 5761 aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt 5821 ctccatttta gcttccttag ctcctgaaaa tctcgacgga tcctaactca aaatccacac 5881 attatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgcgg ccgccaatat 5941 gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt 6001 aatatattga tatttatatc attttacgtt tctcgttcag cttttttgta caaacttgtg 6061 at //