| |
|
Product Name:
|
RVP-1801L, Nipah Virus (NiV) Malaysia/2008, Reporter Virus Particles (RVPs), Renilla Luciferase (RLuc)
|
|
Manufacturer:
|
Integral Molecular Inc., Philadelphia, Pennsylvania, USA
|
|
Additional Information:
|
Reporter virus particles (RVPs) are replication-incompetent, virus-like particles composed of a modified viral minigenome that expresses a reporter gene packaged in virus-specific surface proteins required for entry into a host cell. Depending on the viral type under study, the RVPs may be composed of a heterologous, self-assembling core (such as lentivirus) and native envelope proteins or may be composed of only the structural proteins of the native virus.1
RVPs can serve as live-virus substitutes in infectivity and neutralization assays, allowing for work in a BSL 2 environment. RVPs have applications in screening for neutralizing antibodies and potential drug candidates, conducting large-scale epidemiological studies and aiding in vaccine development.1
Manufacturer's protocols for the use of this item are available on the BEI Resources website.
|
|
Material Provided:
|
Each vial contains approximately 1 mL of RVPs in Dulbecco's Modified Eagle Medium (DMEM) supplemented with 20% fetal bovine serum. The vial should be centrifuged prior to opening.
|
|
Packing/Storage:
|
NR-61718 was packaged aseptically in cryovials. The product is provided frozen on dry ice and should be stored at -80°C or colder immediately upon arrival. Freeze-thaw cycles should be minimized.
|
|
Disclaimers:
|
You are authorized to use this product for research use only. It is not intended for human use. Use of this product is subject to the terms and conditions of the BEI Resources Material Transfer Agreement (MTA). The MTA is available on our Web site at www.beiresources.org. While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this product sheet, neither ATCC® nor the U.S. Government makes any warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. Neither ATCC® nor the U.S. Government warrants that such information has been confirmed to be accurate. This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. ATCC® and the U.S. Government are not liable for any damages or injuries arising from receipt and/ or use of this product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, the U.S. Government, ATCC®, their suppliers and contributors to BEI Resources are not liable for damages arising from the misidentification or misrepresentation of products. |
|
Patents or other restrictions:
|
NR-61718 is claimed in U.S. Provisional Patent Application number US 8691961 B1 and the continuations, continuations-in-part, re-issues, and foreign counterparts thereof.
Recipients of the MATERIAL shall not reverse engineer, make, reproduce, resell or otherwise transfer the MATERIAL to third parties.
|
References:
|
1. Doranz, B., Personal Communication.
2. Li, D., Ye, J. L, Liu, Z. Y. "Generation and Application of a Luciferase Reporter Virus Based on Yellow Fever Virus 17D". Virol Sin. 36 (2021): 1456-1464. PubMed: 34342842.
3. Pierson, T. C., et al. “A Rapid and Quantitative Assay for Measuring Antibody-Mediated Neutralization of West Nile Virus Infection." Virology 346 (2006): 53-65. PubMed: 16325883.
4. Whitbeck, J. C., et al. “Antigenicity, Stability, and Reproducibility of Zika Reporter Virus Particles for Long-Term Applications.” PLoS Negl. Trop. Dis. 18 (2020): e0008730. PubMed: 33206639.
|
|
Citation:
|
Acknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: RVP-1801L, Nipah Virus (NiV) Malaysia/2008, Reporter Virus Particles (RVPs), Renilla Luciferase (RLuc), NR-61718.”
|
|
Biosafety Level:
|
2
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories (BMBL). Current Edition. Washington, DC: U.S. Government Printing Office.
|