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Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
ARP-11402 is a construct consisting of approximately 8000 base pairs including an insertion of the subtype B env gene. The env gene was artificially reconstructed by substituting original viral codons with those of highly expressed human genes without changing the amino acid sequence (GenBank: DQ667594). A Kozak sequence (GCCGCCGCC) was introduced upstream of the ATG start codon. The final construct was ligated into pcDNA3.1(-) (Invitrogen™) expression vector containing genes for ampicillin and neomycin resistance. The size of the env insertion is approximately 2600 base pairs and the translated Env protein is approximately 850 amino acids.
pConBgp160-opt clone and its derivatives, pConBgp120-opt (ARP-11400), pConBgp145-opt (ARP-11401), pConBgp160-201NS-opt (ARP-11403) and pConBgp160-UNC-opt (ARP-11404), can be used for studies of protein expression and function and generation of subunit vaccines against subtype B viruses.
Note: The recommended host strain is JM109. For PCR amplification, it is recommended to use the GC-RICH PCR System (Roche) or another kit specialized for performing difficult amplifications.
Each vial of ARP-11402 contains approximately 10 µg of dried, purified DNA stabilized in DNAstable®Plus (Biomatrica®). Please refer to the appropriate data sheet for lot-specific information.
Kothe, D. L., et al. “Antigenicity and immunogenicity of HIV-1 consensus subtype B envelope glycoproteins.” Virology 360 (2007): 218-234. PubMed: 17097711.
André, S., et al. “Increased Immune Response Elicited by DNA Vaccination with a Synthetic gp120Sequence with Optimized Codon Usage.” J. Virol. 72 (1998): 1497-1503. PubMed: 9445053.
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