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Product Name:
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J820
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Ownership statement:
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This reagent is the tangible property of the U.S. Government.
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Manufacturer:
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BEI Resources
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Taxonomy:
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Bacteria Classification: Alcaligenaceae, Bordetella
Species: Bordetella pertussis
Strain: J820
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Additional Information:
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B. pertussis is a Gram-negative, fastidious, non-motile coccobacilli that is a highly contagious, exclusively human pathogen. It is the causative agent of pertussis (whooping cough), an acute upper respiratory tract infection characterized by coughing fits (paroxysms), a whooping noise heard in the subsequent
inspiration following a paroxysm and prolonged clinical course lasting for several weeks. Infection in adolescents and adults is typically mild; however, in children, particularly young infants,
infection can be severe and sometimes deadly.3,4,5
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Material Provided:
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Each vial contains approximately 0.5 mL of bacterial culture in Bordet Gengou broth supplemented with 10% glycerol.
Note: If homogeneity is required for your intended use, please purify prior to initiating work.
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Packing/Storage:
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NR-59777 was packaged aseptically in cryovials. The product is provided frozen and should be stored at -60°C or colder immediately upon arrival. For long-term storage, the vapor phase of a liquid nitrogen freezer is recommended. Freeze-thaw cycles should be avoided.
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Growth Conditions:
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Media:
Stainer-Scholte broth with Heptakis or Bordet Gengou broth (with 15% rabbit serum) or equivalent
Regan-Lowe agar or Bordet Gengou agar (with 15% rabbit serum) or equivalent
Incubation:
Temperature: 37°C
Atmosphere: Aerobic
Propagation:
1. Keep vial frozen until ready for use, then thaw.
2. Transfer the entire thawed aliquot into a single tube of broth.
3. Use several drops of the suspension to inoculate an agar slant and/or plate.
4. Incubate the tube, slant and/or plate at 37°C for 2 to 7 days.
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Disclaimers:
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You are authorized to use this product for research use only. It is not intended for human use. Use of this product is subject to the terms and conditions of the BEI Resources Material Transfer Agreement (MTA). The MTA is available on our Web site at www.beiresources.org. While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this product sheet, neither ATCC® nor the U.S. Government makes any warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. Neither ATCC® nor the U.S. Government warrants that such information has been confirmed to be accurate. This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. ATCC® and the U.S. Government are not liable for any damages or injuries arising from receipt and/ or use of this product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, the U.S. Government, ATCC®, their suppliers and contributors to BEI Resources are not liable for damages arising from the misidentification or misrepresentation of products. |
References:
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1. Tondella, M. L., Personal Communication.
2. Natraj and, M. S., et al. “Genome-Based Prediction of Cross-Protective, HLA-DR-Presented Epitopes as Putative Vaccine Antigens for Multiple Bordetella species.” Microbiol. Spectr. 12
(2024): e0352723. PubMed: 38054724.
3. Friedman, R. L. “Pertussis: The Disease and New Diagnostic Methods.” Clin. Microbiol. Rev. 1 (1998): 365-376. PubMed: 2906814.
4. Mattoo, S. and J. D. Cherry. “Molecular Pathogenesis, Epidemiology, and Clinical Manifestations of Respiratory Infections Due to Bordetella pertussis and Other Bordetella Subspecies.” Clin. Microbiol. Rev. 18 (2005): 326-382. PubMed: 15831828.
5. Sabella, C. “Pertussis: Old Foe, Persistent Problem.” Cleve. Clin. J. Med. 72 (2005): 601-608. PubMed: 16044656.
6. Weigand, M. R., et al. “The History of Bordetella pertussis Genome Evolution Includes Structural Rearrangement.” J. Bacteriol. 199 (2017): e00806-16. PubMed: 28167525.
7. Weigand, M. R., et al. “Conserved Patterns of Symmetric Inversion in the Genome Evolution of Bordetella Respiratory Pathogens.” mSystems 4 (2019): e00702-19. PubMed: 31744907.
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Citation:
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Acknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Bordetella pertussis, Strain J820, NR-59777.”
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Biosafety Level:
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2
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories (BMBL). Current Edition. Washington, DC: U.S. Government Printing Office.
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