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Product Name:
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2004 Pennsylvania Tomato Outbreak, Serovar Muenchen, Isolate 3
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Manufacturer:
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BEI Resources
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Taxonomy:
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Bacteria Classification: Enterobacteriaceae, Salmonella
Species: Salmonella enterica
Subspecies: Salmonella enterica subsp. enterica1,2
Serogroup: C2-3
Serovar: Muenchen
Isolate: 3
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Additional Information:
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Salmonella enterica (S. enterica) are Gram-negative, rod-shaped, flagellated bacteria. The species is divided into six subspecies (I, II, IIIa, IIIb, IV, VI) where only subspecies I, subsp. enterica, is considered of clinical relevance. Salmonellosis (non-typhoidal), due to the greater than 1500 serovars of S. enterica subsp. enterica is one of the most common food-borne diseases with an estimated 2 million cases that occur in the United States every year.5 Pathogenicity results from a variety of virulence factors found in plasmids, prophages and five pathogenicity islands which allow these organisms to colonize and infect host organisms.6
S. enterica subsp. enterica serovar Muenchen (formerly Salmonella muenchen) is found in domestic and wild animals and is generally spread to humans via consumption of contaminated water or food resulting in gastroenteritis.
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Material Provided:
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Each vial contains approximately 0.5 mL of bacterial culture in Tryptic Soy Broth supplemented with 10% glycerol. Note: If homogeneity is required for your intended use, please purify prior to initiating work. |
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Packing/Storage:
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NR-4313 was packaged aseptically in cryovials. The product is provided frozen and should be stored at -70°C or colder immediately upon arrival. For long-term storage, the vapor phase of a liquid nitrogen freezer is recommended. Freeze-thaw cycles should be avoided.
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Growth Conditions:
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Media:
Tryptic Soy Broth or equivalent
Tryptic Soy Agar or equivalent
Incubation:
Temperature: 37°C
Atmosphere: Aerobic
Propagation:
1. Keep vial frozen until ready for use, then thaw.
2. Transfer the entire thawed aliquot into a single tube of broth.
3. Use several drops of the suspension to inoculate an agar slant and/or plate.
4. Incubate the tubes and plate at 37°C for 1 day.
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Disclaimers:
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You are authorized to use this product for research use only. It is not intended for human use. Use of this product is subject to the terms and conditions of the BEI Resources Material Transfer Agreement (MTA). The MTA is available on our Web site at www.beiresources.org. While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this product sheet, neither ATCC® nor the U.S. Government makes any warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. Neither ATCC® nor the U.S. Government warrants that such information has been confirmed to be accurate. This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. ATCC® and the U.S. Government are not liable for any damages or injuries arising from receipt and/ or use of this product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, the U.S. Government, ATCC®, their suppliers and contributors to BEI Resources are not liable for damages arising from the misidentification or misrepresentation of products. |
References:
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1. Judicial Commission of the International Committee on Systematics of Prokaryotes. “The Type Species of the Genus Salmonella Lignierres 1900 Is Salmonella enterica (ex Kauffmann and Edwards 1952) Le Minor and Popoff 1987, with the Type Strain LT2T, and Conservation of the Epithet enterica in Salmonella enterica over All Earlier Epithets that May Be Applied to This Species. Opinion 80.” Int. J. Syst. Evol. Microbiol. 55 (2005): 519-520. PubMed: 15653929.
2. Tindall, B. J., et al. “Nomenclature and Taxonomy of the Genus Salmonella.” Int. J. Syst. Evol. Microbiol. 55 (2005): 521-524. PubMed: 15653930.
3. Sandt, C. H., et al. “The Key Role of Pulsed-Field Gel Electrophoresis in Investigation of a Large Multiserotype and Multistate Food-Borne Outbreak of Salmonella Infections Centered in Pennsylvania.” J. Clin. Microbiol. 44 (2006): 3208-3212. PubMed: 16954249.
4. Centers for Disease Control and Prevention (CDC). “Outbreaks of Salmonella Infections Associated with Eating Roma Tomatoes--United States and Canada, 2004.” Morb. Mortal. Wkly. Rep. 54 (2005): 325-328. PubMed: 15815562.
5. Altekruse, S. F., M. L. Cohen, and D. L. Swerdlow. “Emerging Foodborne Diseases.” Emerg. Infect. Dis. 3 (1997): 285-293. PubMed: 9284372.
6. Lavigne, J.-P. and A.-B. Blanc-Potard. “Molecular Evolution of Salmonella enterica Serovar Typhimurium and Pathogenic Escherichia coli: From Pathogenesis to Therapeutics.” Infect. Genet. Evol. 8 (2008): 217-226. PubMed: 18226587.
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Citation:
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Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Salmonella enterica subsp. enterica, 2004 Pennsylvania Tomato Outbreak, Serovar Muenchen, Isolate 3, NR-4313."
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Biosafety Level:
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2
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories (BMBL). Current Edition. Washington, DC: U.S. Government Printing Office.
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