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Product Name:
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S11975 (Serovar Newport)
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Manufacturer:
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BEI Resources
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Taxonomy:
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Bacteria Classification: Enterobacteriaceae, Salmonella
Species: Salmonella enterica
Subspecies:Salmonella enterica subsp. enterica
Serovar: Newport
Strain: S11975 (also referred to as strain 11975)1
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Additional Information:
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S. enterica are Gram-negative, rod-shaped, flagellated bacteria. The species is divided into six subspecies (I, II, IIIa, IIIb, IV, VI) where only subspecies I, subsp. enterica, is considered of clinical relevance.3 Salmonellosis (non-typhoidal), due to the greater than 1500 serovars of S. enterica subsp. enterica, is one of the most common food-borne diseases with approximately 1 million cases that occur in the United States every year.4 Pathogenicity results from a variety of virulence factors found in plasmids, prophages, and five pathogenicity islands which allow these organisms to colonize and infect host organisms.5,6
S. enterica subsp. enterica serovar Newport (formerly Salmonella Newport) is prevalent in many geographic regions and has ranked in the top three Salmonella serotypes associated with foodborne outbreaks from 1995 to 2011 in the U.S. It is responsible for several major outbreaks involving tomatoes, ground beef, alfalfa sprouts, and other foods since 2002.7 Dairy cattle have been identified as the main reservoir and antimicrobial resistance is particularly problematic in many serovar Newport strains.8 At least three distinct evolutionary lineages exist in this serotype.7,9
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Material Provided:
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Each vial contains approximately 0.5 mL of bacterial culture in Tryptic Soy broth supplemented with 10% glycerol.
Note: If homogeneity is required for your intended use, please purify prior to initiating work.
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Packing/Storage:
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NR-22087 was packaged aseptically in cryovials. The product is provided frozen and should be stored at -60°C or colder immediately upon arrival. For long-term storage, the vapor phase of a liquid nitrogen freezer is recommended. Freeze-thaw cycles should be avoided.
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Growth Conditions:
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Media:
Tryptic Soy broth or Nutrient broth or equivalent
Tryptic Soy agar or Tryptic Soy agar with 5% defibrinated sheep blood or equivalent
Incubation:
Temperature: 37°C
Atmosphere: Aerobic
Propagation:
1. Keep vial frozen until ready for use, then thaw.
2. Transfer the entire thawed aliquot into a single tube of broth.
3. Use several drops of the suspension to inoculate an agar slant and/or plate.
4. Incubate the tube, slant and/or plate at 37°C for 1 day.
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Disclaimers:
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You are authorized to use this product for research use only. It is not intended for human use. Use of this product is subject to the terms and conditions of the BEI Resources Material Transfer Agreement (MTA). The MTA is available on our Web site at www.beiresources.org. While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this product sheet, neither ATCC® nor the U.S. Government makes any warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. Neither ATCC® nor the U.S. Government warrants that such information has been confirmed to be accurate. This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. ATCC® and the U.S. Government are not liable for any damages or injuries arising from receipt and/ or use of this product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, the U.S. Government, ATCC®, their suppliers and contributors to BEI Resources are not liable for damages arising from the misidentification or misrepresentation of products. |
References:
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1. Davis, M. A., et al. “Multilocus Variable-Number Tandem-Repeat Method for Typing Salmonella enterica Serovar Newport.” J. Clin. Microbiol. 47 (2009): 1934-1938. PubMed: 19386855.
2. Besser, T., Personal Communication.
3. Grimont, P. A. D. and F.-X. Weill. Antigenic Formulae of the Salmonella Serovars, 2007, 9th edition. Paris: WHO Collaborating Centre for Reference and Research on Salmonella, Pasteur Institute.
4. Scallan, E., et al. “Foodborne Illness Acquired in the United States – Major Pathogens.” Emerg. Infect. Dis. 17 (2011): 7-15. PubMed: 21192848.
5. Lavigne, J. P. and A. B. Blanc-Potard. “Molecular Evolution of Salmonella enterica Serovar Typhimurium and Pathogenic Escherichia coli: From Pathogenesis to Therapeutics.” Infect. Genet. Evol. 8 (2008): 217-226. PubMed: 18226587.
6. Parsons, D. A. and F. Heffron. “sciS, an icmF Homolog in Salmonella enterica Serovar Typhimurium, Limits Intracellular Replication and Decreases Virulence.” Infect. Immun. 73 (2005): 4338-4345. PubMed: 15972528.
7. Cao, G., et al. “Phylogenetics and Differentiation of Salmonella Newport Lineages by Whole Genome Sequencing.” PLoS One 8 (2013): e55687. PubMed: 23409020.
8. Berge, A. C. B., J. M. Adaska and W. M. Sischo. “Use of Antibiotic Susceptibility Patterns and Pulsed-Field Gel Electrophoresis to Compare Historic and Contemporary Isolates of Multi-Drug-Resistant Salmonella enterica subsp. enterica Serovar Newport.” Appl. Environ. Microbiol. 70 (2004): 318-323. PubMed: 14711658.
9. Sangal, V., et al. “Evolution and Population Structure of Salmonella enterica Serovar Newport.” J. Bacteriol. 192 (2010): 6465-6476. PubMed: 20935094.
10. Zhang, J., et al. “Whole-Genome Sequences of Four Salmonella enterica Serotype Newport Strains from Humans.” Genome Announc. 1 (2013): e00213-13. PubMed: 23661485.
11. Cao, G., et al. “Draft Genome Sequences of Eight Salmonella enterica Serotype Newport Strains from Diverse Hosts and Locations.” J. Bacteriol. 194 (2012): 5146. PubMed: 22933769.
12. Jacobsen, A., et al. “The Salmonella enterica Pan- genome.” Microb. Ecol. 62 (2011): 487-504. PubMed: 21643699.
13. Yue, M., et al. “Diversification of the Salmonella Fimbriae: A Model of Macro- and Microevolution.” PLoS One 7 (2012): e38596. PubMed: 22701679.
14. Shariat, N., et al. “Subtyping of Salmonella enterica Serovar Newport Outbreak Isolates by CRISPR-MVLST and Determination of the Relationship between CRISPR-MVLST and PFGE Results.” J. Clin. Microbiol. 51 (2013): 2328-2336. PubMed: 23678062.
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Citation:
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Acknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH as part of the Human Microbiome Project: Salmonella enterica subsp. enterica, Strain S11975 (Serovar Newport), NR-22087.”
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Biosafety Level:
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2
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories (BMBL). Current Edition. Washington, DC: U.S. Government Printing Office.
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